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KMID : 0382420020280050028
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Korean Journal of Environmental Health Society
2002 Volume.28 No. 5 p.28 ~ p.34
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A Study on the Antigen Characteristics of Rhodotorula rubra
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Kwon Hyuk-Ku
Lee Jang-Hoon
Ryeom Kon
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Abstract
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Antigenicity of Rhodoltorula rubra isolated from pulmonary tissue of pulmonary tuberculosis patients was studied by means of agglutination reaction with R. rubra whole cell antiserum. And the serological reactivity of crude polysac-from R. rnbra, Candida albicans, Candida tropicalis. Candida glabrata, and Sacchammyces cerevisiae ATCC 26603 with antiserum to R. rubra whole cell was studied by means of immunodiffusion test. R. rnbm showed stationary phase after 48h when it was cultured in GYEP broth. While agglutinogen titer was 1:64 at lag phase, agglutinogen titer was I :256 after 20h. After growth of R. rubm on different 11 media, nutritional environment showed similar agglureactivity. The agglutinogen titer of C. albicans. C. tropicalis, C. glabrata, which were isolated from patient’s expectoration, to R. rubra antiserum by means of agglutination reaction were I: 16. respectively. But, Sacch. cerevisiae ATCC26603 was negative. Those results were lower than that of R. rubm agglutinogen titer I :256. As a result of immufusion test with crude polysaccharide extracted from cell wall of R. rubra, C. albicans, C. tropicalis, C. glabrata, Sacch. cerevisiae ATCC26603, precipitin line was found only with R. rnbra, of which antibody titer was 8.
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